Coding

Part:BBa_K2029002:Design

Designed by: Niklas Schmacke, Max Schelski   Group: iGEM16_UBonn_HBRS   (2016-10-14)


XynA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part codes for the Bacillus subtilis enzyme xynA. It has been sucessfully expressed in B. subtilis strain NZ8900 behind the spaS promoter using plasmid pNZ8902 (ref 1)


Source

Bacillus subtilis

References

1: DOI: 10.1186/1475-2859-11-66, http://www.microbialcellfactories.com/content/11/1/66 2: Kikuchi M., Ogawaa K., Yamazakia T., Kajiwaraa S., Sugiob A., Nakamurab S., Shishidoa K. 1999. Secretional expression of a Bacillus subtilis xylanase gene in the Basidiomycete Coprinus cinereus. FEMS Microbiology Letters, Volume 178, Issue 2, 15 September 1999, Pages 277–282.